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Products > DNA Polymerase and Master Mixes > Pfu DNA Polymerase (Recombinant)

In addition of 5´→3´ DNA Polymerase activity,  Pfu DNA Polymerase also has 3´→5´ exonuclease (proofreading) activity and it is about tenfold more accurate than Taq DNA polymerase and exhibits the lowest error rate of any thermostable DNA polymerase. Pfu Polymerase is useful tool for high fidelity PCR, RT-PCR, Blunt-end PCR cloning.

Reaction buffer:

10XVi Buffer A (without MgCl2): 500mM KCl, 100mM Tris- HCl (pH9.1 at 20oC) and 0.1% TritonTMX100.

10XVi Buffer S: 160mM (NH4)2SO4, 500mM Tris- HCl (pH9.2 at 22oC), 17.5mM MgCl2 and 0.1% tritonTMX-100

3’ to 5’ proofreading activity.

10-fold higher fidelity than Taq.

Recommended for use in high fidelity amplification and cloning blunt.

Normal Pfu -for amplification of up to 8kb (recommended).

Blunt-ended PCR products.

 

PL5201

100U(5 u/µl)   

PL5202

Price PL5201

Price PL5202

500U(5 u/µl)   


 

Price : 0 Rial
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